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Methods Study design and setting Data were obtained from the Netherlands Study of Depression and Anxiety (NESDA), an ongoing naturalistic longitudinal cohort study examining course and consequences of depressive and anxiety disorders

As an unsupervised statistical method, PCA can simplify the multivariate data into a few principal components, which can highlight intrinsic trends, distribution of clusters and the existence of outliers

After treatment, cells were washed, tripsinized and resuspended in FACS buffer (PBS with 5% FBS and 1mM EDTA) and analyzed on an LSR Fortessa (BD Biosciences) with plate reader operated on BD FACSDiva software (v9.0)