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One milligram of total RNA was reverse-transcribed into cDNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche, Indianapolis, IN, USA)

However, preterm infants are highly susceptible to oxidative stress, because they have both increased reactive oxygen species (ROS) formation as well as compromised antioxidant defenses

Note: If you experience a severe allergic reaction, such as swelling of the lips, face, or tongue, trouble breathing, wheezing, or widespread hives, call 911 or go to the nearest emergency room immediately

The homeostasis of iron is also maintained through the regulation of iron absorption, as 1 to 2 mg of iron is lost daily due to skin desquamation, mucous cell sloughing, and blood loss, and the human body has no mechanism to actively remove iron [17]

Each group was randomized by using the website www.randomization.com