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PCR conditions involved an initial denaturation at 98C for 3 min, followed by 30 cycles (98C for 10 s, 55C for 1 min, and 72C for 3 min), concluding with a final extension step of 10 min at 72C

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It consists of a 15 amino acid peptide that covers residues 176-191 of hGH and has a tyrosine added to the N terminus for stabilization

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