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These inhibitors act by specifically reducing free ferrous ions, enhancing antioxidant defenses, inhibiting lipid peroxidation, or indirectly inhibiting ferroptosis through other molecular pathways (Table 1)

Argon et al

Thiol probes to detect Electrophilic natural products based on their mechanism of action

Another study showed that decursin administration reduced CCl4-induced hepatic fibrosis by raising Fe2+ and lipid ROS levels and decreasing GSH and GPX4 levels (Que et al., 2022)

The following antibodies were used: anti CD11b-PE ( 400 diluted using FACS buffer, cat# 553,312: BD Bioscience, Franklin Lakes, NJ), anti Ly6c-FITC ( 100, cat# 553,104: BD Bioscience), anti B220-PE ( 200, cat# 553,309: BD Bioscience), anti CD8alpha-allophycocyanin ( 100, cat# 553,035: BD Bioscience), anti NK1.1-PE ( 100, cat# 553,165: BD Bioscience), anti CD11c-PE ( 100, cat# 557,401: BD Bioscience), anti Ter119-PE ( 40, cat# 125921-83: eBioscience, San Diego, CA), anti CD4-allophycocyanin ( 100, cat# 170042-82: eBioscience, San Diego, CA), anti F4/80-PE ( 40, cat# 124801-80: Invitrogen), and anti CD3-FITC ( 40, cat# 100,305: BioLegend, San Diego, CA)