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The lysates were centrifuged at 17,000 g at 4 C, and 5 L of the supernatant was mixed with 95 L of luminol solution (0.53 M KOH, 1.5% H 2 O 2 , 0.04% luminol)

[DOI] [PMC free article] [PubMed] [Google Scholar] 33.Kietzmann T, Petry A, Shvetsova A, Gerhold JM, Grlach A

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More importantly, the K m for glutathione in the range between 1.5 and 3 mM reveals that the enzymatic action on glutathione is physiologically relevant