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It is the lowest concentration of the analyte that produces a signal that is distinguishable from the background signal or noise

doi: 10.1023/A:1010401015064 77 LeeJ-HLeeMParkJ-ARyuYHLeeKCKimKMet al

At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore

doi: 10.3322/caac.21660 2 ComingsDE