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The seeding density was set at 1 10 4 cells per well, and the plate was incubated for a period of 24 h in RPMI culture medium containing 10% FBS and 1%penicillinstreptomycin in a 37 C incubator with 5% CO 2 and appropriate humidity to allow for cell attachment and growth

Retatrutide has one of the longest plasma half-lives on this peptide half-life chart at approximately 6 days, followed closely by Semaglutide at roughly 7 days and Tirzepatide at about 5 days

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