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Altered complex I activity has been recently shown to increase NADH levels, modulating ROS production by altering electron flow in the ETC (Noch et al, 2024

Gee et al., 2010

Then, the cell viability of HeLa cells was analyzed comparing free MTX and IC by MTT assay (for 72 h), obtaining IC 50 values of 0.0839 and 0.239 mM, respectively