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Passage of bile acids through the enterocyte activates FXR, which promotes the synthesis of fibroblast growth factor 19 (FGF19), which also circulates through the portal vein to the liver where it activates fibroblast growth factor-receptor 4 resulting in inhibition of bile acid synthesis

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A serum blank experiment to detect any interfering peaks in the HPLC chromatogram was performed by in an identical manner except that no drug was added

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