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10.3390/IJERPH18157806 64 TraczJ.HandschuhL.LalowskiM.Marczak.Kostka-JeziornyK.PerekB.et al (2021)

In eukaryotes, an alternative nomenclature is often used using the one-letter code of the amino acid (MARS) and a number is added to refer to the cytosolic (MARS1) or the mitochondrial (MARS2) variants

Backbone and insert fragment were ligated and transformed by heat shock into NEB 5 competent cells following the provided standard procedure of the vendor