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In HOS and MG-63 osteosarcoma cells, the combination of QUE and DOX was shown to reverse DOX resistance in osteosarcoma by increasing mitochondrial biogenesis (51)

And then, the RCA was performed in a volume of 150 L containing 100 L nanoprobe, 5 L CDT, 2 L phi29 DNA polymerase, 15 L 10 phi29 DNA polymerase buffer (330 mM Tris-acetate, pH 7.9 at 37 C, 100 mM Mg-acetate, 660 mM K-acetate , 1% (v/v) Tween 20, 10 mM DTT), 5 L dNTPs and 23 L DEPC-treated water

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Research using kinetic analysis of isotope experiments with tracer doses of radioactive vitamin B12 has shown that the biological half-life of vitamin B12 in plasma is approximately 6 days