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Therefore, a quantitative rate expression for a bimolecular reaction can be given by the following equation: (6) where k exp is the observed second-order rate constant, P A and P B are the partition coefficients of the reactants A and B , respectively

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These changes in the secondary structure brings about large alterations in the tertiary structure of the protein at low pH as evidenced from the gross decrease in the near UV CD and micro-environment of tryptophan (due to hypochromicity with shift in max )