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Flow cytometry analysis of oxidative stress The hPSCs and spheroids were dissociated into a single-cell suspension by incubation in TrypLE Select (Thermo Fisher Scientific, Netherlands) for 5 min with shaking, followed by PBS wash

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Abstract In humans, methionine derived from dietary proteins is necessary for cellular homeostasis and regeneration of sulfur containing pathways, which produce inorganic sulfur species (ISS) along with essential organic sulfur compounds (OSC)

most common at higher doses) [1] [2]