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Description

Publications reported changes in retinal structure or function and proposed that the pineal gland and retina may share aspects of developmental or transcriptional regulation

Briefly, sera or peritoneal fluids (50l) were treated with 10% metaphosphoric acid to precipitate protein (30min, 4C) and then centrifuged for 15min at 18000g at 4C

Tracheotomy was performed and the animals were allowed to breathe spontaneously through the tracheal cannula

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