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Subsequent quantitative reverse transcriptase PCRs were performed using the StepOne Plus RT-PCR system (Applied Biosystems, Waltham, MA, USA) with SYBR Premix Ex Taq II (Takara Bio, Kusatsu, Japan) and primers for amplifying human ALP , VEGFA , and the reference gene GAPDH (see Table 1)

My project aims to explore safer techniques that includes two RNA silencing methods: 1) plant-mediated virus-induced gene silencing (VIGS) and 2) morpholino-mediated gene silencing

International Journal of Medical Sciences , 17 (4), 457470

Intriguingly, the authors hypothesize progesterone amplification of this pathway through PGRMC1, noting E6s selective degradation of nuclear progesterone receptors (sparing membrane isoforms), which may sustain autophagy-mediated immune evasion in HPV oncogenesis [183, 184]