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Seven days after plating, when cell outgrowths had formed, explants were carefully removed by aspiration avoiding the outgrowing cells

RNA Extraction and RT-PCR in the Tendon-Muscle Junction Samples At 7, 14, 28, and 42 postoperative days, TRIzol Reagent (Thermo Scientific, Waltham, MA, USA) was used for extraction, and spectrophotometer (NanoDrop, Thermo Scientific) for quantification at 260 nm of the tissue RNA (Table 1)

Negatively charged nanoparticles and hydrophilic excipients generally demonstrate reduced interaction with mucus, whereas positively charged and hydrophobic agents tend to interact more strongly

Therapie 75, 157160

Curr Drug Discov Technol