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Description

Each larva was placed in a separate well of a 48-well plate containing 1000 l of E3 buffer and kept at 28 C in a dark room to adapt for 30 min

Neuronal differentiation and co-culture with mouse astrocytes Human induced pluripotent stem cells (hiPSCs) from the WTC11 line (for day 28) or PGP1 line (for day 42) were differentiated into cortical glutamatergic neurons following a previously described protocol with modifications 90