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2 Brain organoids: methodologies for generation, types and characteristics 2.1 Methodologies for generation of brain organoids The initial work on the differentiation of 2D cultures (Zhang et al., 2001

This study was funded by the Instituto de Salud Carlos III (ISCIII) through the project PI23/01205 and cofunded by the European Union

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Rectal The rectal route can be used to avoid first-pass metabolism, and may be considered if the oral route is not available