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Reduced states of cobalamin (i.e., +2 and +1) react with dioxygen rapidly to generate a superoxocobalamin.50,51 This species bears analogy to the early intermediates in cytochrome P450 chemistry in which reactive metaloxygen species catalyze the oxidation of pharmaceuticals and other compounds

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To do this, you'll first pull back the plunger to the 1ml mark to draw in air, then inject that air into the BAC water vial