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43 In Phase 1 (removal of embryos), mDANs may die of hypoxic and hypoglycemic insults that occur during embryo removal from the maternal blood supply

Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech) and subsequently transferred to PVDF membrane (Millipore, Temecula) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques

After completion of electrophoresis, the samples were transprinted (semi-dry transfer, 15V for 2h) and blocked (blocked with 5% BSA [Bovine serum albumin] in TBST [Tris-Buffered Saline and Tween 20]), and finally the primary and secondary antibodies (SP1 [Cat., No.: sc-420]