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The indirect antioxidant activity of glutathione is demonstrated by glutathione peroxidase (GPx), which uses glutathione as a reducing agent to catalyze the reduction of H 2 O 2, or organic hydroperoxides to water

Subsequently, cells were incubated with secondary antibodies: PE-conjugated goat anti-mouse IgG (H + L) (for -tubulin, 1:500) and FITC-conjugated goat anti-rabbit IgG (H + L) (for NRF2, 1:500) for 1 h at RT in the dark

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