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Mutations were introduced using QuickChange II site-directed mutagenesis kit (Agilent) and verified by Sanger sequencing

Khamseekaew J, Kumfu S, Palee S, et al

RSL3 mediated cell death, however, was not rescuable by necroptosis inhibitors (Nec-1s) or apoptosis inhibitors (ZVAD-FMK) (Supplementary Fig

Although the immune/inflammatory components of PH require further clarification, recent studies in animal models have shown that anti-inflammatory therapies can reduce or even reverse PH effects