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With four binding sites, NTA binds more strongly to polyhistidine but results in lower yields

In vivo phosphorylation assay The plasmids expressing RBOHD-3HA, RBOHDS22A-3HA, RBOHDT24A-3HA, or RBOHDS22AT24A-3HA were transfected into protoplasts derived from rbohd mutant seedlings followed by incubation overnight in the dark in W5 solution with 40 mCi ml 1 32 P phosphate 66

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Our result for the first time relates a physiological metabolite with the process of gametocytogenesis and implicates homocysteine to be a potential physiological trigger of gametocytogenesis in malaria

& Sarihi, A