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8.1 MSC and apoptosis Nishikawa and the team discovered that the DSS-induced colitis mouse model showed higher concentrations of caspase-3-positive cells and apoptotic nuclear cells in the colon sections and increased colon inflammatory cytokines, leading to decreased intestinal tight junction proteins such as claudin-2 and occludin (253)

RNA was isolated from whole blood as outlined by the Tempus Spin RNA Isolation Kit protocol (Applied Biosystems) and quantified using a NanoDrop (ND-1000 Spectrophotometer)

In a conservative approach, a gene was only identified as differentially expressed if all probes for that gene had a fold change greater than 2 or less than 2 and an ANOVA p -value (Tukey and FDR corrected) less than 0.05

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Commun Biol 7:116