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On the subsequent days spheroids were washed in DMEM/F12 medium (Corning, USA) and the medium was changed, as follows: Basal medium: S1 MCDB131 (Corning, USA), 1% Glutamax (Thermo Fisher Scientific, Netherlands) + 1% Pen/Strep (Thermo Fisher Scientific, Netherlands), 2.44 mM glucose (Merck Millipore, USA), 29 mM sodium bicarbonate (Merck Millipore, USA), 2% BSA (Capricorn Scientific, USA), 2 l/100 mL ITS-X (Thermo Fisher Scientific, Netherlands)

In epilepsy, this interplay is evident in models such as the lithium-pilocarpine (Li-Pilo) model of SE, which recapitulates key features of epileptogenesis including altered glucose metabolism (Dank et al., 2022) and mitochondrial bioenergetics with increased markers of oxidative damage (Carrasco-Pozo et al., 2015

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Review article: oxidative stress as a pathogenic factor in inflammatory bowel disease radicals or ridiculous