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Yeasen Biotechnology, #40734ES75) was applied and mounted with the Antifade Mounting Medium (Beyotime, #P0131)

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Differences in metabolic profiles at baseline and post-treatment were noted between participants who remitted (HRSD 17 7) and those who gained no meaningful benefits ( 14 on HRSD 17 were eligible for inclusion in the trial

All cell lines were grown at 37C with 5% CO 2 in humidified tissue culture incubators (Thermo Fisher Scientific)

Formulated with 0.9% benzyl alcohol as a preservative, this multi-use solution is designed to maintain sterility after initial entry when handled using proper laboratory procedures