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Formula: Volume (mL) = Net Peptide Mass (mg) / Desired Concentration (mg/mL) Example: If your vial contains 2.4 mg of net semaglutide (per CoA) and you want a 1 mg/mL stock: 2.4 mg / 1 mg/mL = 2.4 mL of diluent For a 0.5 mg/mL stock: 2.4 mg / 0.5 mg/mL = 4.8 mL of diluent A 1 mg/mL stock is a common starting point for research, as it can then be serially diluted to nanomolar working concentrations for most in vitro assays

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