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The unspecific proteins were washed in 10 mM NaP i at pH 8, 150 mM NaCl, 1 mM GSH, 10% glycerol, 50 mM imidazole, and 0.1% reduced Triton X-100 (Sigma) and rat MGST1 protein was eluted in 350 mM imidazole

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Any sign suggesting allergic reaction rather than simple skin irritation