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About this article Cite this article Makatjane, K.A., Zhou, H

Cells were detached using 0.25% trypsin-EDTA, collected by centrifugation at a speed of 1000 g for 5 min, and resuspended in PBS

When A monomers aggregate into soluble oligomers, they exert toxic effects on neurons, disrupting cellular signaling and synaptic communication well before they form extracellular plaques (Selkoe and Hardy, 2016