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The content of other critical enzymes in the ASA-GSH cycle, L-1,4-galactonolactone dehydrogenase (GalLDH) and -glutamylcysteine synthetase (-ECS), was determined using assay kits for GalLDH (Solarbio, Beijing, China) and -ECS (D&B, Shanghai, China), based on findings by 2.4 Transcriptome analysis Seedlings exposed to temperatures of 18C, 23C, and 28C for 1 day (early stage of stress), 4 days (mid stage of stress), and 7 days (late stage of stress) were selected for transcriptomic analysis

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As shown in Figure 8, the percentage of NANOG has decreased from 1.37% to 0.011% and 1.19% to 0.011% in OCSCs and BCSCs respectively