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Vitamin E, Vitamin C, Beta carotene, and cognitive function among women with or at risk of cardiovascular disease: The womens antioxidant and cardiovascular study

The protein elution was concentrated with a 100-kDa cut-off concentrator (Millipore) and loaded onto a size-exclusion column (Superose 6 Increase 10/300 GL, Cytiva) equilibrated with buffer C containing 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM DTT, 0.06% digitonin (w/v)

doi: 10.3390/IJERPH19074133 99 BitirgenGKorkmazCZamaniAOzkagniciAZenginNPonirakisGet al

This effect was particularly pronounced in MST6-I cells treated with secretome from TWAT(MST6-I) adipocytes [Supplementary Figure 2D]

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