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The microfluidic device was intended for drug permeability test, and pharmacokinetics (PK) and pharmacodynamics (PD) evaluation whereby the test drug could be delivered either to the upper or bottom channel via a pressure-driven flow to mimic dynamic in vivo conditions, but it could also be adapted to microbiome studies (Kimura et al., 2015)

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Although we did not measure the ROS level directly, the above-described changes in SOD1 may suggest that the M-CFSs of both mushrooms have antioxidant properties in the HT-22 cell line

Notably, metabolites such as AGEs, dysregulated ketone bodies, and BCAAs have been demonstrated to be directly or indirectly associated with structural abnormalities and functional decline in the lung (e.g., impaired surfactant synthesis, diminished respiratory muscle function)