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Phenols, such as hydroxytyrosol and oleuropein, have shown antioxidant properties in vitro and in vivo studies (Posadino et al., 2021)

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Proteins were reduced with 5 mM dithiothreitol (Sigma-Aldrich) for 1 h at RT and alkylated with 10 mM iodoacetamide (Sigma-Aldrich) in the dark for an additional 45 min at RT