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After incubation, the fluorescence intensity of the biofilm was determined at Ex 544 nm and Em 590 nm (Tecan infinite M1000 pro, Sydney Australia)

The laws and penalties for drunk driving began to become harsher in the 1970s through the 1990s, as organizations like Mothers Against Drunk Driving (MADD) and Students Against Destructive Decisions (SADD) began to put pressure on the government to create a lower limit [2]

Collected cells were then resuspended into two different media for hepatic specification following Methods 1 and 2 (Fig

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