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Pharmaceuticals 18, 11 (2024), Abstract TNF modulates PANX1 activation to promote ATP release and enhance P2RX7-mediated antitumor immune responses after chemotherapy in colorectal cancer: K.C.Y

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LC-FL analysis Samples derivatized with monobromobimane (mBB) (Life Technologies) were separated with a Waters Symmetry C18 (ID, 250 4.6 mm) column (Waters Corp., Milford, MA, USA) with mobile phase A(0.25% acetic acid in H 2 O, pH 3.9), B(0.25% acetic acid: methanol = 7:3) and C(0.25% acetic acid: methanol = 1:1) with a linear gradient from A:B = 65:35 to 2:8 in 8 min with a flow rate of 1.5 ml/min, and remained with 100% B for additional 10 min, and then changed to 100% C in the following 6 min with a flow rate of 1.0 ml/min