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5 are likely under-powered: once the timeframe of efficacy for NMN treatment can be established, these experiments could be re-examined using higher experimental power

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At 1 week after the surgery, transthoracic echocardiography (770 VisualSonics Ultrasound System, 17.5 MHz linear array transducer) was implemented under anesthesia induced by 5% isoflurane and maintained by 2% isoflurane in O 2 with activated charcoal absorption filters (VaporGuard)

11 , R834-837 (2001)