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Experimental work, notably by Paulet (1957, 1958), led to the introduction of dicobalt edetate (Kelocyanor)

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In vivo and in vitro experiments clarify the hierarchical regulatory mechanisms: Butyrate directly regulates Th17/Treg homeostasis, promotes Treg differentiation through the HDAC1/FoxP3 pathway, and simultaneously inhibits Th17 activation and the expression of IL-17, IL-23, and RORt (89, 90)

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