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However, NAD + and SIRT3 did not directly interact (Figure 2C), which is consistent with the result of a previous study ( K D of 24.34 M (Figure 2D)

doi: 10.2337/ds18-0002 116 CourtieEVeenithTLoganADennistonAKBlanchRJ

Isothermal titration calorimetry Purified CBS proteins were buffer exchanged into 20 mM HEPES, pH 7.4 using Zeba spin columns (Thermo Fisher Scientific) at 4 C