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Spermine inhibition of monocyte activation and inflammation

doi: 10.1038/s41538-023-00197-z 10

Gel filtration chromatography Recombinant PGRMC1 (a.a.44195) (10 g) wt, Y113F or C129S mutant, treated with 5 mmol l 1 sodium dithionite and/or CO gas or left untreated, was separated on a Superdex 200 column equilibrated in buffer containing 20 mmol l 1 Tris-HCl (pH 7.5) and 100 mmol l 1 NaCl using a SMART system (GE Healthcare)