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& Ryan, D

Harnessing exosomes for the development of brain drug delivery systems

While many worry about its safety during pregnancy, its impact on breastfeeding is also key

doi: 10.1038/s41467-022-31950-9 21 RitchieMEPhipsonBWuDHuYLawCWShiWet al

For loading of the non-hydrolyzable nucleotide analog mant GMPPNP (mGMPPNP, Jena Biosciences), KRAS protein was incubated with alkaline phosphatase beads (Sigma Aldrich) and 20-fold excess nucleotide overnight with gentle rotation at 4C