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Interestingly, the lung GSH (Figure 3B) was maintained at the same level throughout the CS exposure

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Glu cannot cross the blood-brain barrier and is converted in the brain to GABA, an inhibitory neurotransmitter that regulates neural activity, sleep, mood, pain relief, and blood pressure15,16,17 Aspartate/aspartic acid is a powerful antioxidant in the nervous, endocrine, and reproductive systems.18 It supports the synthesis and release of hormones like glucocorticoids, prolactin, oxytocin, and steroids.19 ALT and AST are essential for amino acid conversion and metabolite production

The plates were washed 10 with cold buffer and dried for 15 min at 50 C