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It was originally studied in equine medicine for tendon and muscle recovery, and interest in human therapeutic applications grew as the preclinical data on tissue regeneration, anti-inflammatory effects, and angiogenesis accumulated

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The Kraus 2014 study reported that adipocyte-specific NNMT knockdown in mice increased adipocyte energy expenditure through a mechanism interpreted as elevated methyl-donor availability for polyamine biosynthesis and for histone methylation that modulates adipocyte gene-expression programs [2]

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Animal studies showed fat loss without changing food intake, but it works best combined with healthy eating and activity