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Overall, sulfur-containing amino acid metabolism can be more appropriately viewed as an integrated module of methylation potential and redox homeostasis: the Met-SAM axis is mainly associated with the plasticity of epigenetic and transcriptional programs, the Cys-GSH/H 2 S axis primarily influences oxidative stress and the threshold of inflammatory signaling, and Tau is further linked to antioxidation and the regulation of mitochondrial homeostasis

Reem R, Rosica V, Levinus A D

Mast cells are also located in the spinal cord, and upon activation, the release of mast cell tryptase could directly activate microglia through protease-activated receptor 2 (PAR2), upregulating the synthesis of pro-nociceptive TNF-a and interleukin-6

Shafik NM, Gaber RA, Mohamed DA, Ebeid AM

Regarding instrumental configuration, LC-MS commonly employs reverse-phase chromatographic columns (RPLC), hydrophilic interaction chromatographic columns (HILIC), and ionization methods like electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI)