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Exercise training inhibits inflammation in adipose tissue via both suppression of macrophage infiltration and acceleration of phenotypic switching from M1 to M2 macrophages in high-fat-diet-induced obese mice

Feb 2 2021;10(2)doi:10.3390/antiox10020223

Results Molecular analysis reveals structural stability of GRLX and pKa of individual cysteines Given the previous observations that GLRX can be inactivated via an oxidative mechanism 8,23 , herein we sought to investigate the relative vulnerability of each of the individual cysteines towards GSSG, a model glutathionylating agent, or H 2 O 2 and their contribution to oxidative inactivation

[DOI] [PMC free article] [PubMed] [Google Scholar] 80.Duntas L.H., Benvenga S

Then, the incubated supernatant was added to 20 L of 50% protein A + G-agarose slurry and rotated at 4 C for 2 h