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To perform phospo-protein staining, surface-stained cells were fixed with BD cytofix fixation buffer (BD Biosciences), followed by permeabilization with BD Phosflow Perm Buffer III (BD Biosciences) and pSTAT5 or pS6 antibody staining

The highest and most complex level encapsulates impaired cellular metabolism , which maximally impacts neurons in the hallmark neurodegenerative regions [42,43,44,45], but also affects other metabolically active non-neuronal cells throughout the brain and body, such as glia and myocytes [46,47,48]

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Insights into oxidative stress in bone tissue and novel challenges for biomaterials

Oxidative damage and protection of the RPE