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8c, Supplementary Data 13 and 15)

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Morphological studies on cataract and small lens formation in neonatal rats treated with monosodium-L-glutamate

Key Findings: Single injections produced dose-dependent increases in GH concentrations of 2- to 10-fold lasting 6 or more days IGF-1 levels increased 1.5- to 3-fold for 911 days after a single dose Multiple doses produced cumulative effects, with IGF-1 remaining elevated above baseline for up to 28 days No serious adverse reactions were reported The peptide was safe and relatively well tolerated, particularly at doses of 30 or 60 g/kg Animal Research: A study published in the American Journal of Physiology demonstrated that once-daily administration of CJC-1295 normalized growth in GH-deficient models, supporting its potential therapeutic applications

For purification, 2030 g of cells were resuspended in lysis buffer comprised of 2xTBS (40 mM Tris-HCl pH 8.0, 300 mM NaCl) and supplemented with 10 mM benzamidine, 10 mM 6-aminocaproic acid and 1 mM PMSF