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c) To confirm SILAC data, SKOV3 cells, seeded on T25 flasks, were transfected with plasmids pTagRFP-C-SRSF4 and pEGFP-C1-TIA1 131 using Lipofectamine 3000 (Invitrogen) according to the manufacturers protocol

Rayman MP

Materials and methods Database analysis The differential expression of C5aR1 between glioma tissues and adjacent noncancerous tissues was analyzed using the Cancer Genome Atlas (TCGA