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An assay of glutathione peroxidase activity in tissue homogenates was performed at 37C in a reaction medium containing Tris-HCl buffer supplemented with tetrasodium ethylenediaminetetraacetate and sodium azide (pH 8.5) in the presence of 0.55 mM reduced glutathione and 0.192 mM hydrogen peroxide

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Intestinal CYP2E1-mediated oxidative stress can also sensitize the liver to toxicity through endotoxemia and gut-derived TNF- through the CYP2E1-thioredoxin-ASK1-JNK1 pathway [203]