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Microscopy SEM was done with a SUPRA 40VP (Carl Zeiss MicroImaging) equipped with a K1250X Cryogenic SEM Preparation System (EMITECH), a CPD 030 critical point dryer (Bal-Tec) and a SC 7600 sputter coater (Polaron) at the on-campus microscopy core facility Zentrale Mikroskopie (CeMic) of the Max Planck Institute for Plant Breeding Research, Cologne

All fluorescence images were imaged by an UltraView Vox Spinning Disk Confocal Microscope (PerkinElmer) (63X)

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First strand cDNA was synthesised from 1 g of total RNA with miRNA 1st Stand cDNA Synthesis Kit (by stem-loop) (Vazyme Biotech co

84 Nevertheless, the results from the studies are conflicting, and sufficiently large genomic studies are needed to further develop the understanding