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Tissue sections were blocked with 3% goat serum for 60 minutes at room temperature, stained with an antibody cocktail solution (containing 750 g/mL of each antibody) at 4C for 15 hours, washed with 0.2 % Triton X-100 in PBS for 8 minutes, and then stained with nuclear Intercalator-Ir (Fluidigm) at 300 L/section

[599] underscore the need for large-scale targeted mechanistic studies, such as longitudinal gut-microbiome profiling, in vivo models of -synuclein propagation, and integrated immunemetabolite analyses, to elucidate pathways along the gutbrain axis

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The goal of supervised tapering is harm reduction and symptom minimisation rather than complete symptom prevention

Lifespan and healthspan extension by resveratrol and related dietary stilbenes: Mechanisms and potential